Fig 1. Aspn^-/- mouse generation.

(A) Strategy for the creation of Aspn-null allele. The diagram shows wild-type, targeted, floxed, and excised alleles. Exons 1–4 (out of 8 present in the gene) are shown as rectangles. Primers used for genotyping are targeted to P1, P2, P3 sites and the expected PCR products’ sizes are denoted. (B) Genotyping PCR of Aspn^-/- mice using primers targeted to sites P1, P2, P3. (C) Confirmation of Aspn protein expression loss in Aspn^-/- mice by immunobloting tail SDS extracts for asporin.