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. 2017 Aug 31;12(8):e0184028. doi: 10.1371/journal.pone.0184028

Fig 3. Skin glycosaminoglycan (GAG) analysis.

Fig 3

GAGs were purified from two-month old dorsal skin (n = 5 per genotype) and digested with chondroitinase ABC or chondroitinase B or a mixture of heparinases. The resulting disaccharides were fluorescently labeled, separated by HPLC and quantified. (A) Total GAGs are the sum of chondroitin/dermatan sulfate (CS/DS), hyaluronic acid (HA), and heparan sulfate (HS). (B) Compositional analysis of CS/DS chains. UA = unsaturated hexuronic acid obtained after chondroitinases digestion, either unsulfated or 2-O-sulfated (UA-2S); GalNAc = N-acetyl-galactosamine either unsulfated or 4-O-sulfated (GalNAc-4S). (C) Compositional analysis of HS chains. The measured HS disaccharides were grouped according to the sulfation position: non-sulfated = UA-GlcNAc; N-sulfated = UA-GlcNS + UA-2S-GlcNS + UA-GlcNS-6S + UA-2S-GlcNS-6S; 2-O-sulfated = UA-2S-GlcNAc + UA-2S-GlcNAc-6S + UA-2S-GlcNS + UA-2S-GlcNS-6S; 6-O-sulfated = UA-GlcNAc-6S + UA-2S-GlcNAc-6S + UA-GlcNS-6S + UA-2S-GlcNS-6S. UA = unsaturated hexuronic acid obtained after heparinases digestion, either unsulfated or 2-O-sulfated (UA-2S); Glc = glucosamine either N-acetylated (GlcNAc) or N-sulfated (GlcNS) which can be both O-unsulfated or 6-O-sulfated (GlcNAc-6S and GlcNS-6S). Error bars show standard deviation.