Table 2. Quantification of gene transcripts using qPCR.
| Relative transcript amount in Aspn-/- (fold of wild-type) |
Wild-type | Aspn-/- | ||||
|---|---|---|---|---|---|---|
| p value | dCt | sd dCt | dCt | sd dCt | ||
| Col1a1 | 6.86 | 0.00004 | -0.21 | 0.71 | -2.99 | 0.27 |
| Col1a2 | 3.96 | 0.00004 | 2.63 | 0.28 | 0.64 | 0.46 |
| Col3a1 | 3.52 | 0.00008 | 1.85 | 0.37 | 0.03 | 0.41 |
| Fmod | 1.57 | 0.3 | 6.82 | 1.03 | 6.17 | 0.87 |
| Lox | 3.52 | 0.0002 | 5.73 | 0.34 | 3.91 | 0.54 |
| Loxl2 | 1.41 | 0.07 | 5.28 | 0.45 | 4.78 | 0.28 |
| Plod2 | 0.23 | 0.004 | 5.32 | 0.18 | 7.43 | 1.19 |
| Lum | 2.21 | 0.03 | 7.68 | 0.87 | 6.54 | 0.47 |
| Fbn1 | 1.02 | 0.9 | 3.91 | 0.57 | 3.88 | 0.16 |
| Mmp2 | 2.41 | 0.002 | 4.15 | 0.47 | 2.88 | 0.38 |
| Mmp3 | 3.19 | 0.0005 | 7.87 | 0.50 | 6.19 | 0.45 |
| Serpinh1 | 2.68 | 0.0001 | 5.36 | 0.31 | 3.94 | 0.35 |
| Mia3 | 1.91 | 0.001 | 8.81 | 0.31 | 7.88 | 0.31 |
Dorsal skin RNA from two-month old female mice (n = 5 per genotype) was used to determine mRNA levels of selected extracellular matrix-related genes. Aspn-/- mRNA levels are presented as percentage of wild-type mRNA levels, all normalized to Actb. P values were calculated using Student’s unpaired two-tailed t-test. Also shown are delta Ct (dCt) and standard deviation (sd dCt).