Fig 5. miR129-5p expression in hESC-derived cardiomyocytes with hypoxia.

A) hESC-derived cardiomyocytes were cultured in 1% O₂ and relative expression of vascular endothelial growth factor A (VEGFA) and miR129-5p were assessed by qPCR. Upregulation of hypoxia-mediated VEGFA and downregulation of miR129-5p was seen. Data shown are mean±SEM (n = 3); *p<0.05. B) To demonstrate viability of hESC-derived cardiomyocytes grown in 1% O2, cells were stained with trypan blue and percent live cells (trypan blue-excluding) were calculated. Hypoxia did not significantly affect cell viability. Data shown are mean±SEM (n = 3).