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. 2017 Aug 31;12(8):e0183624. doi: 10.1371/journal.pone.0183624

Fig 6. Bone morphogenetic protein receptor 2 is a target of miR129-5p in HL1 cells.

Fig 6

A) In silico analysis of potential miR129-5p targets using Target Scan Human identified bone morphogenetic protein receptor 2 (BMPR2) as a potential target. Conservation among mammals of the miR129-5p binding site in the 3’UTR of BMPR2 is shown below. Bta, cow; Cfa, dog; Cpo, guinea pig; Eca, horse; Fca, cat; Hsa, human; Laf, elephant; Mml, rhesus; Mmu, mouse; Ocu, rabbit; Ptr, chimpanzee; Rno, rat; Sar, shrew. B) HL1 cells transfected with BMPR2 3’UTR reporter (REP) show increased luciferase activity in 1% O2 and 100 μM H2O2. Co-transfection with miR129-5p (mimic) downregulates luciferase activity. Reporter plasmid expressing luciferase with scrambled BMPR2 3’UTR sequence (REPscr) was used as control. Data shown are mean±SEM (n = 3); *p < 0.05; **p<0.01. C) HL1 cells were grown in 1% O2 or 100 μM H2O2 and relative expression of BMPR2 in the presence and absence of transfected miR129-5p (mimic) was assessed by qPCR. An increase in BMPR2 expression was seen under both conditions of oxidative stress, and this increase was suppressed by overexpression of miR129-5p. Data shown are mean±SEM (n = 3); *p<0.05; **p<0.01.