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. 2017 Aug 31;12(8):e0184088. doi: 10.1371/journal.pone.0184088

Fig 2. Peptide B-8R induces apoptosis and blocks tumorigenesis of prostate cancer cells.

Fig 2

LNCaP or PC-3 cells were treated with Vehicle (0) or 10–25 μM Peptide B-8R, as shown, for 0–24 hrs and subjected to (A, B) Western blotting to measure either (A) Pro-Caspase 3 or (C) PARP cleavage or to (B) Caspase 3 assay, all of which monitored apoptosis. (D) LNCaP cells were treated with Vehicle or 50 μM Peptide B-8R for 1 hr and subjected to Annexin V/PI dual staining for apoptosis. Bar graphs represent averages of three independent experiments plus standard deviations. Asterisks indicate statistical significance (P<0.005) of Peptide B-8R activity, relative to Vehicle (0 μM). Note that β-actin was used to control for protein loading.