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. 2017 Aug 31;12(8):e0184088. doi: 10.1371/journal.pone.0184088

Fig 3. Peptide B-8R interacts with sGCα1 in prostate cancer cells.

Fig 3

(A) LNCaP cells were treated with Biotin-labeled Peptide A-8R and subjected to immunocytochemistry using anti-sGCα1 or anti-Biotin antibody to measure subcellular co-localization of endogenous sGCα1 and Peptide B-8R. DAPI was used to stain nuclei. (B) LNCaP cytosolic extracts were incubated with Peptide B-8R-Biotin, plus or minus a 20-fold excess of unlabeled Peptide B-8R or C-8R, and subjected to purification using streptavidin-agarose. Western blotting was used to measure the co-purification of sGCα1. Asterisks indicate statistical significance (P<0.02) of Peptide B activity, relative to Vehicle.