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. 2017 Aug 21;13(8):e1006873. doi: 10.1371/journal.pgen.1006873

Fig 2. Effect of Rvb2 depletion on Mec1 and Tel1 functions.

Fig 2

(A) Expression of Rvb2-aid after AID activation. Cultures of rvb2-aid cells were analyzed as in Fig 1A. (B) Cell proliferation after Rvb2 depletion. Cultures of rvb2-aid cells were monitored as in Fig 1B. (C) Expression levels of endogenous Mec1 or Tel1 protein after Rvb2 depletion. rvb2-aid cells expressing Mec1-FLAG or Tel1-FLAG were analyzed as in Fig 1C. (D) Rad53 phosphorylation after Rvb2 depletion. rvb2-aid cells expressing Rad53-HA were examined as in Fig 1E. (E) Levels of MEC1 or TEL1 mRNA after Rvb2 depletion. rvb2-aid cells were analyzed as in Fig 1D.