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. 2017 Aug 21;13(8):e1006873. doi: 10.1371/journal.pgen.1006873

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© 2017 Goto et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Interaction of Asa1 with Mec1 and Tel1. Cells expressing Asa1-myc and Mec1-HA or Tel1-HA were analyzed as in Fig 3B. (B) Expression of Asa1-aid after AID activation. Cultures of asa1-aid cells were analyzed as in Fig 1A. (C) Cell proliferation after Asa1 depletion. Cultures of asa1-aid cells were examined as in Fig 1B. (D) Expression levels of endogenous Mec1 or Tel1 protein after Asa1 depletion. asa1-aid cells expressing Mec1-FLAG or Tel1-FLAG were analyzed as in Fig 1C. (E) Levels of MEC1 or TEL1 mRNA after Asa1 depletion. asa1-aid cells were analyzed as in Fig 1D. (F) Rad53 phosphorylation after Asa1 depletion. asa1-aid cells expressing Rad53-HA were examined as in Fig 1E. (G) Effect of Asa1 depletion on newly synthesized Mec1 and Tel1 proteins. asa1-aid cells, carrying the GAL-FLAG-MEC1 or the GAL-FLAG-TEL1 plasmid, were cultured and analyzed as in Fig 1F. (H) Effect of Asa1 depletion on pre-synthesized Mec1 and Tel1 proteins. asa1-aid cells, carrying the GAL-FLAG-MEC1 or the GAL-FLAG-TEL1 plasmid, were cultured and analyzed as in Fig 1G.