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. 2017 Aug 21;13(8):e1006873. doi: 10.1371/journal.pgen.1006873

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© 2017 Goto et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — (A, B, C) Cells expressing Tel2-HA (A), Pih1-myc (B) or Asa1-HA (C) were grown to mid log-phase and spheroplasted. Spheroplasts were homogenized to prepare whole-cell extracts (W) and then separated into the cytoplasmic (C) and nuclear (N) fractions. Samples from each fraction were separated by SDS-PAGE and immunoblotted with anti-HA, anti-myc, anti-Zwf1 (Glucose-6-Phosphate Dehydrogenase; G6PDH) or anti-nuclear pore complex (NPC) antibodies. (D) Two different Tel2 pathways and protein localization. See the text.