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. 2017 Aug 21;11(8):e0005861. doi: 10.1371/journal.pntd.0005861

Fig 2. Phenotypic and functional characterization of the RosaCre-/+ IL-4Rα-/lox deletable mouse model.

Fig 2

A. IL-4Rα GMFI of total cells from several organs 5 days following Tamoxifen administration summarized in B. C. Total cell numbers in several organs. D. IL-4Rα GMFI of major immune cells taken from several organs. E. Kinetics of IL-4Rα GMFI change over time following Tamoxifen treatment of RosaCre-/+ IL-4Rα-/lox mice. A relative IL-4Rα expression of 100% was assigned to IL-4Rα-/lox mice and a relative expression of 0% was assigned to IL-4Rα-/- mice. Do note the similarity in IL-4Rα expression levels between IL-4Rα-/lox and oil-treated RosaCre-/+ IL-4Rα-/lox control mice. F. Long-term effect of Tamoxifen gavage on the body weight of RosaCre-/+ IL-4Rα-/lox mice. G. Upregulation of surface expression of IL-4Rα by spleen lymphoid cells stimulated with rIL-4. Cells were collected 48 hours following incubation with rIL-4 and IL-4Rα mean surface expression on CD3+ T (H) and CD19+ B cells (I) was determined and plotted. Each experiment was conducted at least twice with 3–6 mice per group. Data are expressed as mean ± SD; NS = p > 0.05; * = p < 0.05; ** = p < 0.01; *** =, p < 0.001; **** = p < 0.0001.