Table 2. Bacterial strains and plasmids.
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| S. gordonii | ||
| M99 | endocarditis causing parental strain | [37] |
| PS1225 | M99 expressing gspB736flag | [20] |
| PS1244 | PS1225 Δasp2; SpecR | [20] |
| PS3539 | PS1225 asp2S362A | This study |
| PS3309 | PS1225 Δgly-nss; SpecR | This study |
| PS3540 | PS3539 Δgly-nss, asp2S362A; SpecR | This study |
| PS921 | M99 expressing gspB1060flag | [10] |
| PS3349 | PS921 Δasp2; SpecR | This study |
| PS3541 | PS921 asp2S362A | This study |
| PS3318 | PS921 Δgly-nss; SpecR | This study |
| PS3542 | PS3541 Δgly-nss asp2S362A; SpecR | This study |
| PS1290 | PS1225 ΔgtfA; SpecR | This study |
| PS3549 | PS1225 ΔgtfA, asp2S362A; SpecR | This study |
| PS1064 | PS921 ΔgtfA; SpecR | This study |
| PS3550 | PS921 ΔgtfA, asp2S362A; SpecR | This study |
| PS3551 | PS1225 asp2E452A | This study |
| PS3552 | PS1225 asp2H482A | This study |
| PS3553 | PS1225 asp2S362AE452A | This study |
| PS3554 | PS1225 asp2S362AE452AH482A | This study |
| PS3555 | PS921 asp2E452A | This study |
| PS3556 | PS921 asp2H482A | This study |
| PS3557 | PS921 asp2 S362AE452A | This study |
| PS3558 | PS921 asp2S362AE452AH482A | This study |
| PS3584 | PS1225 Δasp2,Δgly-nss; CmR, SpecR | This study |
| PS1226 | PS1225 ΔsecA2 | [38] |
| PS3311 | PS1225 Δgly-Δasp3; SpecR | This study |
| PS3310 | PS1226 Δgly-nss; SpecR | This study |
| PS3605 | PS1226 Δgly-nss asp2S362A; SpecR | This study |
| PS922 | PS921 ΔsecA2 | [10] |
| PS3323 | PS922 Δgly-Δasp3; SpecR | This study |
| PS3322 | PS922 Δgly-nss; SpecR | This study |
| PS3606 | PS922 Δgly-nss asp2S362A; SpecR | This study |
| PS3316 | PS1225 gspB736flag L75A79C80 Δgly-nss, ΔsecA2; SpecR | This study |
| PS3317 | PS1225 gspB736flag L75A79C80 Δgly-Δasp3; SpecR | This study |
| PS666 | M99 ΔgtfA; SpecR | [12] |
| PS3319 | M99 Δgly-nss; SpecR | This study |
| PS3536 | M99 asp2S362A | This study |
| E. coli | ||
| TOP10 | Host cell for cloning | Invitrogen |
| BL21 | Host cell for protein expression | Novagen |
| PS875 | E. coli expressing glycosylated GST-SRR1 | [25] |
| Plasmids | ||
| pCOLA.H6asp123 | E. coli expression vector encoding asp123 KnR | This study |
| pMalE-asp2-H6 | E. coli expression vector encoding MalE-asp2 fusion protein; AmpR | This study |
| pMalE-asp2S362A-H6 | E. coli expression vector encoding MalE-asp2S362A fusion protein; AmpR | This study |
| pGLYNSSK | pS326 carrying upstream fragments of gly and downstream fragments of nss; SpecR | This study |
| pGLYNSSK | pS326 carrying upstream fragments of gly and downstream fragments of asp3; SpecR | This study |
CmR, chloramphenicol resistant
ErmR, erythromycin resistant
SpecR, spectinomycin resistant