Fig. 2.

CL mutants are not sensitive to paraquat, menadione or H₂O₂. Cells were cultured at 30°C in rich media to the early stationary phase, serially diluted and spotted on YPD (A) and YPE (1% ethanol) (B) plates supplemented with 0.5 mM paraquat, 0.1 mM menadione or 2.5 mM H₂O₂. The plates were incubated at 37°C. While crd1Δ and taz1Δ cells are sensitive to 1% ethanol in liquid media, sensitivity on solid media is detectable at concentrations greater than 2%.