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. 2017 Aug 15;6:e28111. doi: 10.7554/eLife.28111

Figure 1. Phylogenetic analysis of Beaten Path and Sidestep paralogs.

(A) Phylogeny of the Beat family of receptors rooted against the tick Ixoides scapularis (Ixo) Beats. Beat-VII and Beat-VI share a more recent ancestor than previously described. (B) Extracellular architecture of the Side subfamily. Detailed ClustalW alignment of individual domains and conservation are in Figure 1—figure supplement 1. (IG, immunoglobulin superfamily; FNIII, Fibronectin type III; TM, transmembrane domain). (C) Phylogeny of the Side family of related proteins rooted against similar IgSF proteins predicted in the tick, Ixoides scapularis (Ixo) that form a distinct outgroup. Names are assigned to the paralogs on the basis of their evolutionary distance from Sidestep and their CG Flybase identifiers are in parentheses.

Figure 1.

Figure 1—figure supplement 1. Sequence alignments of extracellular domains in the Side family.

Figure 1—figure supplement 1.

Alignment of the five IgSF domains (Ig) and the fibronectin type III domain (FN III) for the different Side family members. Alignments were generated with ClustalOmega with the individual IgSF or FNIII domains of Side, Side-II, III, IV, V, VI, VII and VIII (GenBank accession numbers: AAF56708.2, AAX52666.3, ABI31143.1, AAF54952.3, AAF46967.2, ABI31162.1, AGB95814.1 and AAF57468.4 respectively). Domain boundaries are predicted by an initial search with SMART and extended by homology modeling using PHYRE2 and secondary structure predictions using PSIPRED. Positions conserved in the IgSF or FNIII fold are shadowed in black if they present a very high degree of conservation in the fold (residues corresponding to positions B3, C5 and F3 in the IgSF domains and C1 and F1 in the FNIII domains respectively) or grey if they present a high degree of conservation in the fold (residues corresponding to positions B1, C3, E5 and F5 in the IgSF domains and C3, C5, F3 and F5 in the FNIII domains respectively).