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. 2017 Aug 15;6:e28111. doi: 10.7554/eLife.28111

Figure 7. Embryonic expression of beat-I and beat-V subgroups in motor neurons indicates regulatory divergence.

Figure 7.

Fluorescent in situ mRNA hybridization for the beat-I and beat-V groups of genes (magenta). Individual motor neurons are marked (green) with anti-myc in a Lim3A-tau-myc (Lim3) line and anti-myc or anti-βGal in RN2-Gal4 > UAS tau-myc-eGFP and RN2-Gal4 > UAS LacZ (RN2) to reveal RP1, 3, 4, and 5 (Lim3) or aCC and RP2 (RN2) cells, respectively. (A) beat-Ia is expressed in the ISN pioneer motor neurons aCC and RP2 and in the ISNb motor neurons RP1, 3, 4, and 5 (arrowheads). (B) beat-Ib is not expressed at observable levels in the RP1, 3, 4 or 5. (C) beat-Ib is expressed only at low levels (isolated magenta dots) in aCC and RP2. (D) beat-Ic is not expressed in RP1, 3, 4 and 5, but is expressed (E) in aCC and RP2. (G) beat-Va is not expressed in RP1, 3, 4, or 5 motor neurons but is expressed in aCC and RP2 (H), clearly showing higher levels in RP2 than in aCC. (I) beat-Vb is expressed at high levels in RP1, 3, 4 and 5 motor neurons and at low levels in aCC and RP2 (J). All embryos are dissected to expose the CNS. Anterior is up in all images, with the ventral midline in the center. Coordinates of orthogonal slices are indicated on main panels and XY and XZ cuts are represented to the right and bottom of each panel respectively. (F) Expression profiles of the beat-I and beat-V genes in dorsally (aCC, RP2) and ventrally (RP1, 3, 4, 5) projecting motor neurons. Scale bars indicate distances.