Skip to main content
. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Dev Cell. 2017 Aug 7;42(3):213–225.e4. doi: 10.1016/j.devcel.2017.07.009

Figure 2. Samd14-Enh Confers Survival in Severe Anemia.

Figure 2

(A) Kaplan-Meier survival curve of WT and Samd14-Enh-/- mice following two doses (one per day) of 60 mg/kg PHZ (n = 16 in each group). (B) Spleen weight of WT and Samd14-Enh-/- mice 3 days after treatment with vehicle (PBS) or PHZ (100 mg/kg). (C) Hematologic parameters of WT (n = 6) and Samd14-Enh-/- (n = 6) mice following PHZ treatment (100 mg/kg). (D) Quantification of Burst Forming Unit-Erythroid (BFU-E) colonies 5 days after plating splenic cells (1 × 105) from mice treated with PHZ for 0, 24, 48, or 72 hours (h) in methylcellulose containing Epo, SCF, IL-3, and IL-6. (E) Quantification of BFU-E colonies 5 days after plating, and Colony Forming Unit-Erythroid (CFU-E) colonies 2 days after plating, 1 × 105 cells from mice treated with PHZ for 0 or 72 h in Epo-containing methylcellulose. Statistical significance represented by mean +/- SEM.; *p<0.05. **p<0.01, ***p<0.001.