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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Dev Cell. 2017 Aug 7;42(3):213–225.e4. doi: 10.1016/j.devcel.2017.07.009

Figure 5. Samd14-Enh Promotes Anemia-Dependent SCF/c-Kit Signaling.

Figure 5

(A) Flow cytometric analysis of Ter119-depleted WT control spleen stained with CD71 and c-Kit (left) and quantitation of pAKT in the CD71+Kit+ cell population. All histograms were normalized to the percentage of the maximal cell number. (B) Flow cytometric analysis of WT PHZ-treated spleen stained with CD71 and c-Kit (top left) and histograms of pAKT in CD71+Kit- cells (bottom left), and pAKT and pERK in CD71+Kit+ cells (right). (C) Flow cytometric analysis of WT and Samd14-Enh-/- PHZ-treated spleen stained with CD71 and c-Kit (left) and comparative histogram plots of pAKT and pERK in WT and Samd14-Enh-/- CD71+Kit+ SCF-stimulated cells (right). (D) Quantitation of pAKT and pERK MFI in WT and Samd14-Enh-/- cells (n = 3). Statistical significance represented by mean +/- SEM.; *p<0.05; **p<0.01.