Table 2.
Characteristics of the current known interactions between the FA proteins and non-FA proteins
| Protein | FANC proteins | Identification mechanism | Organism/Model System | Functional Significance |
|---|---|---|---|---|
|
| ||||
| ATM | FANCD2 | IF, IVA | H. sapiens | IR-inducible S phase checkpoint, IR-inducible FANCD2 phosphorylation |
| FANCI | IP | H. sapiens | ND | |
|
| ||||
| ATR | FANCD2 | IF, IVA | H. sapiens | DNA damage-inducible FANCD2 mono-ubiquitination and nuclear foci formation, FANCD2 phosphorylation |
| FANCG | ND | H. sapiens | ND | |
| FANCI | IP | H. sapiens | ND | |
|
| ||||
| BLM | FANCA | IP | H. sapiens | DNA crosslink repair, DNA replication fork repair, FANCC, FANCD2, and FANCG required for efficient MMC-inducible localization of BLM to nuclear foci |
| FANCD2 | IF, IP | H. sapiens, G. gallus | ||
|
| ||||
| BRCA1 | FANCD1/BRCA2 | IF, IP | H. sapiens, M. musculus | Mitotic and meiotic recombination, DNA crosslink repair |
| FANCD2 | IF, IP | H. sapiens | IR- and UV-inducible FANCD2 mono-ubiquitination and nuclear foci formation | |
| FANCA | IP, Y2H | H. sapiens | ND | |
| FANCJ/BRIP1 | FW, IF, IP, GST-PD | H. sapiens | Homologous recombination, DNA crosslink repair | |
|
| ||||
| BRG1 | FANCA | Y2H, IP, IF | H. sapiens | ND |
|
| ||||
| CHK1 | FANCE | IVA | H. sapiens | DNA damage-inducible FANCE phosphorylation and FANCD2 mono-ubiquitination, DNA crosslink repair |
|
| ||||
| CLK2 | FANCD2 | ND | H. sapiens, C. elegans | DNA damage-inducible FANCD2 mono-ubiquitination and nuclear foci formation, DNA crosslink repair |
|
| ||||
| H2AX | FANCD2 | IF, IP | H. sapiens, M. musculus | DNA damage-inducible FANCD2 nuclear foci formation, Chromatin re-modeling, DNA crosslink repair |
|
| ||||
| HHR6 | FANCD2 | ND | H. sapiens | DNA damage-inducible FANCD2 mono-ubiquitination, DNA crosslink repair |
|
| ||||
| NBS1 | FANCD2 | IP, IF | H. sapiens | IR and ICL-inducible S phase checkpoints, IR-inducible FANCD2 phosphorylation, DNA crosslink repair |
|
| ||||
| PCNA | FANCD2 | IF, IP, IVA | H. sapiens | FANCD2 mono-ubiquitination and nuclear foci formation, DNA crosslink repair |
|
| ||||
| RAD51 | FANCD2 | IF | H. sapiens | ND |
| FANCD1/BRCA2 | IF, Y2H, GST-PD | H. sapiens, M. musculus, C. elegans, U. maydis | FANCD1/BRCA2 Promotes RAD51 nucleoprotein filament formation and promotes RAD51-mediated DNA strand exchange | |
|
| ||||
| REV1 | FANCA | ND | H. sapiens, G. gallus | FANCA and FANCG promote REV1 nuclear foci formation and REV1-dependent TLS |
| FANCG | ND | |||
| FANCD2 | IF | ND | ||
|
| ||||
| RPA | FANCD2 | IF | H. sapiens | DNA damage-inducible FANCD2 mono-ubiquitination and nuclear foci formation, DNA crosslink repair |
|
| ||||
| TIP60 | FANCD2 | Y2H, GST-PD, IP, IF | H. sapiens | Chromatin re-modeling, DNA crosslink repair |
|
| ||||
| UBE2T | FANCL | Y2H, GST-PD, IVA | H. sapiens, G. gallus | E2 ubiquitin-conjugating enzyme, promotes FANCD2 mono-ubiquitination |
|
| ||||
| USP1 | FANCD2 | IP, IVA | H. sapiens, G. gallus | De-ubiquitination of mono-ubiquitinated FANCD2, DNA crosslink repair |
Key: GST-PD, GST pull-down assay. FW, Far western. IF, immunofluorescence microscopy. IP, Immunoprecipitation. IVAIn vitro assay. ND, Not determined. TLS, Translesion DNA synthesis. Y2H, Yeast two-hybrid.