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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Cell Syst. 2017 Jan 25;4(2):242–250.e4. doi: 10.1016/j.cels.2017.01.002

Fig. 2. Endocytic and retromer trafficking proteins interact with α-syn physically and genetically.

Fig. 2

(A) Endocytic and retromer trafficking proteins enriched in α-syn-APEX2-labeled neurons are tested for physical interaction with a-syn using the membrane-2-hybrid (MYTH) technique (Fig. S2). Interactions scored as positive are marked “+.” Positive and negative controls are shown in the box. (B) Spotting assays on yeast agar plates indicate that deleting each of the three core retromer components (VPS26, VPS29 and VPS35) enhances toxicity in a strain expressing moderately toxic levels of α-syn (IntTox) but does not enhance toxicity in Control or NoTox α-syn yeast strains.