Table 1. Summary of the relationship of cytosine deamination rate with incubation temperature and incubation time with the bisulphite–hydroquinone solution.

Temperature (°C)	1 h	n	4 h	n	18 h	n
0	98.00	1 (10)	78.30 ± 25.03	2 (20)	82.80	1 (11)
15	88.60 ± 3.68	2 (24)	98.35 ± 0.49	2 (22)	54.25 ± 11.24	2 (18)
35	30.05 ± 0.07	2 (19)	69.75 ± 1.34	2 (21)	94.10 ± 5.09	2 (21)
55	77.30 ± 2.40	2 (23)	99.48 ± 0.39	4 (41)	99.65 ± 0.52	4 (45)
80	92.69 ± 9.22	2 (23)	–	–	–	–
85	94.65 ± 0.49	2 (24)	93.20 ± 9.62	2 (19)	93.40 ± 0.32	2 (24)
95	98.05 ± 2.20	5 (53)	–	–	–	–

The ratio of deaminated cytosine is given as a percentage of total cytosine content in between the primer binding sites of the upper strand of ARTα. n indicates the number of independent experiments that yielded a PCR product. The total number of sequenced clones is given in parentheses.