Fig. 8.

Structure of DPPC/POPC aLUVs above and below the melting temperature of DPPC via a joint analysis of SAXS and SANS data using headgroup (dH) and hydrocarbon (dC) deuterated lipid variants. At low temperatures (a) DPPC-rich gel-like domains coexist on the outer leaflet with a fluid POPC-rich phase. The area per lipid is significantly larger than in symmetric DPPC bilayers (0.47 nm²). The gel-like domains melt upon raising temperature to 50 °C (b). The larger content of POPC on the inner leaflet leads to a slightly larger average area per lipid as compared to the outer leaflet. Figure adapted from Heberle et al. (2016) and Eicher et al. (2017)