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. 2017 Aug 31;7:10152. doi: 10.1038/s41598-017-09232-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PLIN1 mRNA levels and CpG-methylation in obese and never-obese women from the explorative cohort. (a) PLIN1 mRNA in WAT of obese (n = 9) and never-obese (n = 9) women. PLIN1 mRNA quantification was performed with microarray. (b) Average methylation of several CpG-sites in the PLIN1 gene assessed by microarray in adipocytes from obese (n = 15) and never-obese (n = 14) women. (c) Methylation of cg04998447 in adipocytes from obese (n = 5) and never-obese (n = 9) women. (d) Methylation of cg01035422 in adipocytes from obese (n = 5) and never-obese (n = 9) women. Quantification of CpG methylation in c. and d. was performed with Pyrosequencing. Values are mean ± SD. **P < 0.01.