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. 2017 Aug 31;7:10216. doi: 10.1038/s41598-017-10487-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic workflow for packing and analysis of N-glycan using AutoTip. The AutoTip uses pipette tips with capacity from 20–300 μL. (a) A polyethylene sheet was inserted into the AutoTip, followed by loading Aminolink resin (200 μL) before sealing AutoTip by another polyethylene sheet. The AutoTip is operated by an automatic liquid handler (e.g., Thermo Versette); (b) Glycan analysis: immobilization of protein to AutoTip, modification of glycan, and release of N-glycans. 96-well plate is used for sample preparation.