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. 2017 Aug 31;7:10216. doi: 10.1038/s41598-017-10487-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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N-glycan profile of prostate cancer urines by MALDI-MS. (a) Heat map of 21 N-glycans from 58 prostate cancer urines. Three groups were characterized by AutoTip using a liquid handler, including 38 Gleason 6, 15 Gleason 7, and 6 Gleason 9. The abundance of N-glycans were normalized using mean value of all N-glycans, and heatmap was plotted after being logarithmized. (b) Pseudo-3D plot of N-glycans of 58 prostate cancer urines. The sialylated N-glycans were highly abundant in urines. (c) Cartoon structures representing the likely isoform of 22 N-glycan compositions identified in urines.