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. 2017 Aug 31;7:10229. doi: 10.1038/s41598-017-10323-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Depletion of SIRT6 suppresses osteogenic differentiation in vitro. (A) Western blotting was conducted to examine the knockdown efficiency of SIRT6. (B,C) SIRT6 depletion decreased ALP activity as determined by ALP staining (B), and ALP quantification assay when cells were cultured in osteogenic media for 7 days (C). (D,E) SIRT6 depletion impaired mineralization as determined by Alizarin Red S staining (D) and quantification (E) when cells were treated with osteogenic media for 14 days. (F–H) SIRT6 knockdown inhibited the expressions of BGLAP (F), SPP1 (G) and RUNX2 (H), as determined by quantitative real-time RT-PCR. All data are shown as mean ± SD, n = 3. **P < 0.01 and ***P < 0.001. pm: proliferation-inducing media; om: osteogenic-inducing media.