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. 2017 Aug 31;7:10272. doi: 10.1038/s41598-017-09880-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gonadotropin enhances sKitlg expression in the antral follicles and restores folliculogenesis in DHT-treated rats. (A) Forty eight hours after eCG injection (10 IU, i.p.), ovaries from CTL and DHT-treated rats were sectioned and stained with hematoxylin and eosin. eCG increased of ovarian size and antral follicle number in the DHT -treated rats. (B) After eCG injection (10 IU, 48 h), antral follicles isolated from CTL and DHT-treated rats were lysed and soluble-kit ligand mRNA abundances were assessed by quantitative RT-PCR. Results are expressed as mean ± SEM (n = 3). Data were analyzed by 2-way ANOVA and Bonferroni post-hoc test. CTL group: **P < 0.01 (vs. eCG); DHT group: **P < 0.01 (vs. eCG). (C) After eCG injection, ovarian tissue sections from CTL and DHT-treated rats were immunostained for soluble-kit ligand, analyzed and quantified using the ImageJ software. Results are expressed as mean ± SEM of 15 follicles from 3 experimental replicates. Data were analyzed by 2-way ANOVA and Bonferroni post-hoc test. DHT group: **P < 0.01 (vs. eCG); Without eCG group: **P < 0.01 (CLT vs. DHT).