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. 2017 Aug 31;7:10213. doi: 10.1038/s41598-017-10222-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The 455UCOE region remains as an active chromatin domain compared to the 527UCOE region in murine P19 cells. Enrichment of the active and repressive histone marks and the chromodomain protein MPP8 on the 455UCOE and the 527UCOE region was assessed by ChIP assay in transduced P19 cells at day 10 post transduction. The value of enrichment for each antibody is determined relative to the input, IgG and then normalized to the actively transcribed GAPDH or to the repressive NGN1 locus. The normalized values of the enrichment at the 527UCOE, and the 455UCOE are shown. The data represents 2–3 independent ChIP assays. P values shown are determined by the t-test (two tailed).