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. 2017 Aug 31;7:10217. doi: 10.1038/s41598-017-10687-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Swap mutations across the predicted salt bridge D335-K464. (a) Relative positions of D335 (red) and K464 (blue) in the Fzo1 model. The green and pink regions correspond to the N- and C-terminal halves, respectively (Supplementary Fig. 9). (b) Dextrose and glycerol growth spot assay. (c) Anti-Fzo1 and anti-Pgk1 immunoblots of whole-cell extracts prepared from the strains analysed in (b). Molecular weight markers are indicated on the right. (d) Anti-Myc and Anti-Pgk1 immunoblots of whole-cell extracts prepared from the indicated strains. Molecular weight markers are indicated on the right of long or short exposures of the immunoblots.