Figure 4.
CaV1.2 internalization in rat cardiomyocytes treated with AngII. Representative confocal images of CaV1.2 immunofluorescence in rat cardiomyocytes, control (A) or treated with AngII (1 µM) for 1 hr without (B) or with (C) losartan (100 nM). All fluorescence images were collected at the same gain setting of the microscope, nucleus stained with DAPI. (D) Intracellular CaV1.2 immunofluorescence staining intensity measurements normalized to control cardiomyocytes (n = 6–10 for each condition) from cardiomyocytes treated with AngII (1 µM) for different time points. *p < 0.01 with respect to control.