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. 2017 Aug 31;7:10243. doi: 10.1038/s41598-017-08452-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 17β-estradiol and ICI on THRSP expression in primary hepatocytes. (A,B and C) APOV1, MC5R and THRSP mRNA expression levels quantified by qPCR in primary hepatocytes treated by different doses of 17β-estradiol. (D,E and F) APOV1, MC5R and THRSP mRNA expression levels in primary hepatocytes treated by 100 nM 17β-estradiol and 10 nM ICI. Each data point represents the mean ± SD of 6 chickens (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; NS, no significant difference versus control.