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. 2017 Aug 31;7:10243. doi: 10.1038/s41598-017-08452-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 17β-estradiol on TH relative genes expression levels in the liver. (A–F) THRα, THRβ, Dio1, Dio2, ADH1B and PIK3CA mRNA expression levels in chicken liver quantified by qPCR after they were treated with different doses of estrogen for 12 hours, respectively. Each data point represents the mean ± SD of 6 chickens (n = 6). *P < 0.05; **P < 0.01; NS, no significant difference versus control.