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. 2017 Aug 31;7:10270. doi: 10.1038/s41598-017-10619-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and distribution of Atg16L1 after SE. (A) Western blotting analysis of Atg16L1 expression in the hippocampus of SE rats. Equal levels of protein samples (20 μg) were loaded, and β-actin served as a loading control. The relative optical density (Atg16L1/β-actin) were analyzed and represented as the mean ± SD (n = 3; **p < 0.01, compared with the ctrl group). (B) The cell type specificity of Atg16L1 was performed with double fluorescent immunolabeling (TRITC-labeled, red), accompanied with the astrocyte-specific marker GFAP (FITC-labeled, green). The nucleus was stained by DAPI (blue). Scale bar = 50 μm. The percent of GFAP and Atg16L1 positive cells was analyzed (n = 5; ns, no significant difference compared with the ctrl group). (C) The cell type specificity of Atg16L1 was performed with double fluorescent immunolabeling (TRITC-labeled, red), accompanied with the GFAP (FITC-labeled, green). The nucleus was stained by DAPI (blue). Scale bar = 50 μm. The percent of GFAP and Atg16L1 positive cells was analyzed (n = 5; **p < 0.01, compared with the ctrl group). (D) The cell type specificity of Atg16L1 was performed with double fluorescent immunolabeling (TRITC-labeled, red), accompanied with the neuron-specific marker NeuN (FITC-labeled, green). The nucleus was stained by DAPI (blue). Scale bar = 50 μm. The percent of NeuN and Atg16L1 positive cells was analyzed (n = 5; **p < 0.01, compared with the ctrl group).