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. 2017 Aug 31;7:10167. doi: 10.1038/s41598-017-10862-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of CYPs on the response of mouse ORs to acetophenone. The effects of CYPs, CYP1a2 (Yellow), CYP2a5 (Green), CYP2f2 (Light blue), CYP2b10 (Blue), CYP2b19 (Purple), CYP2g1 (Red) and CYP2j6 (Grey), on the response of ORs to acetophenone were compared with the control (without CYP, White). The ORs M71, MOR123-2, MOR161-2, MOR264-5, MOR184-1 and MOR164-2 were used for this analysis. The responses were analyzed using the Dual-Glo luciferase assay system as described in the Materials and Methods. The relative response of ORs was calculated as (Luc/RL)/(Luc/RL without odorant solution). The means ± standard deviation of three separate experiments are shown. The statistical significance was assessed using a t test (*P < 0.05, **P < 0.01).