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. 2017 Aug 9;4(8):170325. doi: 10.1098/rsos.170325

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Fluorescamine method is compatible with DMSO and HFIP. (a) Standard curve using hen egg white lysozyme in the presence of 70% DMSO (open and closed circles) and 70% HFIP (open and closed triangles). The presence of 70% TFA completely abolished fluorescamine reaction (open and closed squares). Lines are linear fits to the data. (b) Concentration of Aβ42 determined using the fluorescamine method in the absence and presence of 70% DMSO or 70% HFIP. Error bars are the standard deviations of three independent measurements.