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. 2017 Aug 31;7:10134. doi: 10.1038/s41598-017-09452-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PEDF deficiency had limited effect on long-term hematopoiesis and HSC function in secondary transplantations. (A) Engraftment, percent CD45.2 + cells, and lineage distribution (B) in PB of secondary recipients 4 weeks post transplant. (C) The graphs show engraftment, proportion of CD45.2 cells, in total BM and lineage distribution (D) 16 weeks post transplant in BM of secondary transplanted mice. (E) Graph shows FACS analysis of LSK cells in BM 16 weeks after secondary transplantation. Results shown are the mean ± SEM from three independent experiments (n = 3 donors per genotype and 2–3 recipients per donor).