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. 2017 Aug 31;7:10134. doi: 10.1038/s41598-017-09452-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dose response curve for recombinant human PEDF in human HSPCs. PEDF-dose response curve for CD34 + cord blood cells. (A) Cells were cultured in SFEM media supplemented with SCF, TPO and Flt3L, all cytokines in 100 ng/ml with or without recombinant human PEDF at given concentrations (n = 3). (B) CD34 + cells were cultured in SFEM supplemented with 25 ng/ml SCF with or without recombinant human PEDF at given concentrations (n = 2–4). (C) After five days of culture with or without recombinant PEDF in SFEM media (supplemented with SCF, TPO and Flt3L) cells were plated in methylcellulose media. Graph shows number of colonies per 100 cells plated (n = 4). (D) Graph shows percentage of cells expressing CD34 or apoptotic markers after five days of culture in SFEM (supplemented with SCF, TPO and Flt3L) (n = 2). Results shown are the mean ± SEM.