Figure 4.

Quantification of NF1Δ37 using a boundary spanning probe (A) or a boundary spanning primer (B). (A) Equal amounts of water (red curves) or NF1+37 (blue curves) were added. Equal C_tvalues are obtained for the first three dilutions. Influence on amplification efficiency from the presence of full-length transcript is already observed for dilution 4 by a reduction of end-point fluorescence and an increase in C_tvalue for the blue curve. At higher C_tvalues quantification is no longer reliable as can be seen for the C_t difference between dilutions 5. (B) No difference is seen between the series with the addition of water (red) and NF1+37 (blue).