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. 2017 Aug 31;7:10188. doi: 10.1038/s41598-017-10684-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of EBV genome in PBL-1. Genetic map of EBV is shown (left). Deleted region of PBL-1 is indicated by the red arrow. Deleted region in B95.8 EBV is indicated by the lower black arrow. The right panels show results of PCR analysis for EBV DNA encoding BHRF1 and BART miRNA clusters (upper panel), and PCR of the deletion site of EBV in PBL-1 (lower panel). Upper blue lines in the EBV genetic map indicate PCR amplicons for EBV DNA encoding miRNAs in the right upper panel. Lower blue arrows in EBV genetic map are PCR primers used for PCR analysis of the breakpoint (right lower panel).