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. 2017 Aug 31;7:10202. doi: 10.1038/s41598-017-09725-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of co-immunoprecipitation with anti-LdMAPK1 polyclonal antibody (A) before and (B) after treatment with geldanamycin. LdL: L. donovani whole cell lysate, PIP: co-immunoprecipitate with preimmune sera, CIP: co-immunoprecipitate with anti-LdMAPK1 sera. Proteins were separated on SDS-PAGE and analyzed by western blotting with anti-LdMAPK1, anti-HSP90, anti-HSP70, anti-STI1 and anti-SGT antibodies, separately.