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. 2017 Aug 31;7:10164. doi: 10.1038/s41598-017-10755-7

Figure 2.

Figure 2

Activity of recombinant LysEF-P10 and its two domains. (A) Bactericidal activity. Log(CFU/ml) decrease in the E. faecalis N10 culture (108 CFU/ml) was used to evaluate the bactericidal activity of native LysEF-P10 (20 µg/ml), LysEF-P10C (100 µg/ml), LysEF-P10B (100 µg/ml), and LysEF-P10 (20 µg/ml) pre-treated with EDTA (100 mM). As a control, N10 was treated with an equivalent quantity of Tris-HCl buffer. Error bars = ±SDs (n = 3). (B) Binding activity of LysEF-P10B-GFP. E. faecalis N10 was dyed with 20 μM/l Hoechst No. 33342 at 37 °C for 10 min and incubated with LysEF-P10B-GFP at 37 °C for 10 min. (1) Localization at 405 nm (blue, emitted by Hoechst No. 33342). (2) Localization at 488 nm (green, emitted by GFP). (3) Image with normal light. (4) Overlay of (1), (2), and (3). The bars indicate 2 μm.