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. 2017 Aug 31;7:10164. doi: 10.1038/s41598-017-10755-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of LysEF-P10-specific antibodies. (A) Titres of total antibodies. Serum samples from LysEF-P10-treated (5 µg) mice were collected every week for 8 weeks. The concentrations of total antibodies were measured using ELISA. (B) Titres of IgG, IgM, and IgE isotypes. Concentrations of IgG (filled circles), IgM (filled squares), and IgE (filled triangles) isotypes were measured using ELISA. (C) Influence of anti-LysEF-P10 serum on the bactericidal activity of LysEF-P10. LysEF-P10 was pre-incubated with serum from normal mice (filled squares) or mice treated with 5 µg LysEF-P10 once (filled triangles) or immunized with 50 µg LysEF-P10 thrice (inverted filled triangles) for 10 min. The three mixtures or buffer (filled circles) alone were added to cultures of VREF E. faecalis E028. The log(CFU/ml) decrease in the E028 culture (10⁸ CFU/ml) was used to evaluate the bactericidal activity at various time points, as indicated. Values represent means ± SDs (n = 3).