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. 2017 Aug 31;7:10180. doi: 10.1038/s41598-017-10465-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dexamethasone enhances anti-GRα binding to the distal IL1RL1 gene promoter in the rs6543115(C)/rs6543116(A) haplotype. (A) Schematic representation of GREs in the IL1RL1 distal and proximal promoters, with the GRE position given in reference to Exon 1a (+1) for the distal promoter and Exon 1b (+1) for the proximal promoter. Boxes indicate the specific GRE sequence in each promoter. (B) ChIP assays for HMC-1 human mast cells stimulated with 100 nM Dex using an Ab57-specific polyclonal antibody against GRα, (C) an antibody against RNA pol II or control normal serum (IgG). The % input represents the % DNA precipitated using the specific antibody. n = 4. Kruskal-Wallis test with Dunn’s multiple comparison post-test. **p < 0.01; ***p < 0.001. (D) Luciferase activity associated with a 1211-bp region corresponding to the IL1RL1 distal promoter containing the three putative GREs (GREs 1, 2 and 3) in haplotypes rs6543115(C)/rs6543116(A) (pIL1RL1(Hap1)-Luc) or rs6543115(G)/rs6543116 (G) (pIL1RL1(Hap2)-Luc, as determined in A549 cells treated with 0–1000 nM Dex. (E) Schematic luciferase reporter plasmids generated from pIL1RL1(Hap1)-Luc and percentage of luciferase activity induced by 100 nM Dex. Luciferase activity was normalised to Renilla (F/R = firefly/Renilla activity) and related to the control. Error bars represent the means ± SEM of 3 experiments. Two-way ANOVA. **p < 0.01; ***p < 0.001.