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. 2017 Aug 31;7:10212. doi: 10.1038/s41598-017-10634-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antagonistic interaction between JA and cytokinin in xylem development. (a) Root growth of Col-0 grown in the indicated conditions for 7 days (MeJA, 10 μM MeJA; cytokinin, 50 nM BAP; MeJA/cytokinin, 10 μM MeJA and 50 nM BAP). (b) Quantification of the number of xylem cells in the xylem axis of these plants (n > 20). (c) Vasculature morphology of Col-0 grown in the indicated conditions for 7 days. Black arrows and arrowheads indicate extra xylem cells and phloem cells, respectively. The yellow dotted line indicates a xylem axis. (d) Quantification of extra xylem formation in these plants. Percentages were calculated by dividing the number of plants with extra xylem by the number of plants observed. Scale bar = 0.5 cm in (a) and 20 μm in (c).