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. 2017 Aug 31;7:10212. doi: 10.1038/s41598-017-10634-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gradual suppression of the cytokinin response by JA. (a) Suppression of the cytokinin response by JA was analyzed in a time course by monitoring fluorescent signals in ARR5::GFP plants. Five-day-old ARR5::GFP plants grown without JA were transferred to MS media containing 10 μM MeJA, and grown for the indicated times. Mock indicates MS media containing 0.05% EtOH. White arrows and arrowheads indicate cytokinin response in the vascular tissue and root cap, respectively. (b) Quantification of extra xylem formation in these plants (n > 20). Percentages were calculated by dividing the number of plants with extra xylem by the number of plants observed. Scale bar = 20 μm.