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. 2017 Aug 31;7:10212. doi: 10.1038/s41598-017-10634-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of the cytokinin response promotes the formation of extra xylem. (a) Cross section images of 35S::AHP6 roots grown in MS media for 7 days. (b) Quantification of extra xylem formation in these plants (n > 30). Percentages were calculated by dividing the number of plants with extra xylem by the number of plants observed. (c) Expression levels of the JA-induced genes LOX2 and JR2 in these plants. Line 1 and 2 indicate individual lines of 35S::AHP6 transgenic plants. Error bars represent S.D. GAPDH was used as a reference gene. Black arrows indicate extra xylem cells. Scale bar = 20 μm.