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. 2017 Aug 31;7:10212. doi: 10.1038/s41598-017-10634-1

Figure 6.

Figure 6

JA responses in plants with reduced cytokinin responses. LOX2 (a) and JR2 (b) expression was analyzed in 35S::AHP6 plants by qRT-PCR. Seven-day-old 35S::AHP6 and Col-0 seedlings were transferred to media containing 100 μM MeJA and incubated for the indicated time. Total RNA was extracted from these roots. Expression levels of LOX2 (c) and JR2 (d) in two independent lines of 7-day-old 35S::AHP6 plants treated with 100 μM JA for 6 hrs. (e) Root growth of 35S::AHP6 plants grown in JA-treated and -untreated conditions for 7 days (n > 30). (f) Quantification of extra xylem formation in these plants (n > 29). Percentages were calculated by dividing the number of plants with extra xylem by the number of plants observed. (g) Root vasculature (top) and high-resolution images (bottom) of wol mutants grown in 10 μM MeJA-untreated (left) or -treated (right) conditions for 12 days. (h) Expression levels of LOX2 and JR2 in wol mutant roots. Ten-day-old wol and Col-0 seedlings were transferred to MS media containing 100 μM MeJA and incubated for 3 hrs. Total RNA was extracted from the roots of these plants. GAPDH was used as a reference gene. Error bars represent S.D. and asterisks indicate statistically significant differences between the corresponding samples and their control (p < 0.01, t-test). Scale bar = 10 μm.