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. 2017 Aug 31;7:10212. doi: 10.1038/s41598-017-10634-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AHP6 and MYC2 are involved in JA-mediated xylem development. (a) Root vasculature of ahp6-1 plants grown in 10 μM MeJA-untreated (top) or -treated (bottom) conditions for 7 days. The red dotted line indicates a xylem axis. (b) Quantification of extra xylem formation in the roots of Col-0, ahp6-1, and myc2-3 mutants grown in 10 μM MeJA or not treated for 7 days (n > 22). (c and d) The number of vascular tissues cells (c) and xylem cells (d) in JA-treated and -untreated ahp6-1 mutant plants. (e) Analysis of AHP6 expression in myc2-3 mutants by qRT-PCR. Total RNA was extracted from Col-0 and myc2-3 roots grown in 10 μM MeJA or not treated for 7 days. GAPDH was used as a reference gene. Error bars represent S.D. and asterisks indicate statistically significant differences between the corresponding samples and their control (p < 0.01, t-test). (f) GUS staining of AHP6::GUS/myc2-3 plants grown in 10 μM MeJA or not treated for 7 days using 1 mM ferrocyanide/ferricyanide-containing GUS staining solution. Scale bar = 20 μm in (a) and 100 μm (d).