Figure EV1. Rad51‐dependent DSB repair occurs before metaphase I.

1. hop2Δ strains in the BR1919 background were transferred to sporulation media. At 22 h (hop2Δ rad17Δ) or 30 h (all other strains), cells were harvested and meiotic chromosomes were spread for immunofluorescence microscopy as in Fig 4B. Scale bar, 5 μm.
2. Quantification of the results in (A). The percentage of nuclei with metaphase spindle was determined for each strain; this represents cells that have exit pachytene and progressed into metaphase I. From nuclei with metaphase spindles, the percentage that are positive for DNA damage markers was determined; this represents cells that have progressed into metaphase I with unrepaired DSBs (e.g., the hop2Δ rad17Δ strain, in which the DNA damage checkpoint gene RAD17 has been deleted). NA, not applicable. At least 150 nuclei were scored for each strain.