Figure 2. The Cdc5‐Dbf4 fusion protein can suppress pachytene arrest.

Either CDC5 or the CDC5‐dbf4‐R83E fusion construct was placed under the control of the DBF4 promoter and integrated at an ectopic locus (URA3) in the indicated strains. “–” denotes no ectopic insert.

1. Strains were induced to synchronously enter meiosis. Proteins were detected, and cell cycle kinetics was monitored as in Fig 1B.
2. Cells were incubated for 48 h on sporulation plates, and sporulation percentage was determined by light microscopy. White labels depict the native DBF4 locus, and gray labels depict the ectopic locus.

Data information: Data in (A) and (B) are represented as means from two and three experiments, respectively. Error bars in (B) indicate ± SEM. At least 100 cells were scored per experiment.Source data are available online for this figure.