Figure 5. Cdc5 kinase activity is required for destruction of SC components, unshackling of Rad51, and phosphorylation of Dbf4.

• A, B
  Strains were induced to synchronously enter meiosis. At 6 h, the culture was split and either carrier (Cdc5 induction −) or β‐estradiol (Cdc5 induction +) was added. Cells were harvested at the indicated time points to detect proteins as in Fig 1B (A) or monitor meiotic chromosomes as in Fig 3A (B). Cells are from the same cultures.
• C, D
  Strains were induced to synchronously enter meiosis. At 6 h, β‐estradiol was added to induce production of Cdc5 or Cdc5‐kd (kinase dead, Cdc5‐N209A). Cells were harvested at the indicated time points to detect proteins as in Fig 1B (C) or monitor meiotic chromosomes as in Fig 3A (D). Cells from the cdc5‐kd set of strains are from the same cultures. “−” denotes the absence of an inducible CDC5 or cdc5‐kd allele at the URA3 locus.
• E
  Strains were induced to synchronously enter meiosis. At 6 h, β‐estradiol was added to induce production of Cdc5. Cells were harvested at 10 h and resolved by SDS–PAGE in gels containing the indicated amounts of Phos‐tag reagent. Cells are from the same cultures as Fig 4A. “−” denotes the absence of an inducible CDC5 allele at the URA3 locus.

Data information: Data in (B) are represented as the results of an individual experiment (see Fig D for the duplicate experiment). Data in (D) are represented as means from two experiments.Source data are available online for this figure.